Genetic Manipulation of Agrobacterium

Soybean: Plant Manipulation to Agrobacterium Mediated Transformation

Revolution in plant biotechnology can be categorized into in vitro culture and genetic transformation. Plant regeneration was successfully achieved in 1950’s while production of transgenic plant was accomplished in 80’s. For production of transformants, in vitro culturing strategies are prerequisite. Soybean has been cultured through organogenesis and embryogenesis but still it is considered re...

Genetic Manipulation of Tomato (lycopersicon Esculentum) Using Wga Gene through Agrobacterium Mediated Transformation

The major problems faced in the field of Agriculture are loss in crop yield caused by insects, Herbs (weeds), viruses and the pathogens and the pests associated loss is about 14% of total agricultural production. The use of pesticides has resulted in adverse effect on the beneficial organisms and other plant parts such as leaves, fruits and has reached pollution levels, which has become a major...

Agrobacterium: nature’s genetic engineer

Agrobacterium was identified as the agent causing the plant tumor, crown gall over 100 years ago. Since then, studies have resulted in many surprising observations. Armin Braun demonstrated that Agrobacterium infected cells had unusual nutritional properties, and that the bacterium was necessary to start the infection but not for continued tumor development. He developed the concept of a tumor ...

Genetic transformation of HeLa cells by Agrobacterium.

Agrobacterium tumefaciens is a soil phytopathogen that elicits neoplastic growths on the host plant species. In nature, however, Agrobacterium also may encounter organisms belonging to other kingdoms such as insects and animals that feed on the infected plants. Can Agrobacterium, then, also infect animal cells? Here, we report that Agrobacterium attaches to and genetically transforms several ty...

Endophytic Fungi, Characteristics and Their Potential for Genetic Manipulation